Highly efficient depletion for an RNA-seq application using an innovative benchtop platform for workflow automation and miniaturization.
In this study, the benefits of using an rRNA depletion application in Magelia® were evaluated for RNA-seq. Equal amounts of human control total RNA (100 ng) were treated in parallel manually and in the Magelia®, using Illumina’s Ribo-Zero Plus rRNA Depletion Kit.
Highlights include:
Improved reaction kinetics allowed for more efficient enzymatic rRNA removal.
Lower counts of rRNA reads were found for Magelia® treated samples, underlying improved depletion.
Improved transcriptome depth was seen for Magelia® treated samples, highlighting key advantages of the platform’s technology.
Precise magnetic bead manipulation resulted in minimal material loss.
Biological concordance was established by verifying the transcriptional status of the top, and bottom 20 transcribed genes reported on the Harmonizome for the studied human cell line.
Full RNA-seq library prep is now available in the Magelia®.